Recombinant Proteins
P-1006 Recombinant Protein A/GRecombinant Protein A/G is a genetically-engineered protein that consists of five Fc binding domains from protein A (EDABC) and two from protein G (C2C3), resulting in a final mass of 50.5 kDa by SDS-PAGE. The protein A portion is from Staphylococcus aureus and the protein G portion from Streptococcus. The protein A/G (EDABC-C2C3) is expressed in and purified from E. coli. Its binding capacity of Protein A/G is broader than either Protein A or Protein G. It binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. In addition, its binding to IgG is less pH-dependent than Protein A, occurring well at pH 5 - 8. Its extended Fc-binding properties and lack of binding to serum albumin make it a popular tool in the investigation and purification of immunoglobulins.
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