Recombinant Proteins
P-1002 Recombinant Tetracycline Repressor (TetR)The recombinant Tetracycline Repressor (Rec. TetR) of class B with a C-terminal 6-his tag is expressed in bacteria and purified primarily as previously described by Reichheld et al*. It has a m.w. of approximately 23 kDa.
Tetracycline (tc) is a broad family of antibiotics to which bacterial have evolved resistance. The expression of tc resistance genes is regulated by tetracycline repressor protein, called TetR. The tet regulatory system is widely used for selective target gene regulation in ukaryotic cells. The TetR can be used in studies on gene regulations associated with mRNA and protein expression in cell–free expression system+. In addition, the TetR can also be used as a tc binding receptor in assays designed to quantitatively or semi-quantitatively measure tetracycline and its related antibiotics, such as competitive ELISA or immunochromatograph-based lateral flow.
* Eeichheld SE et al. The induction of folding cooperativity by ligand binding drives the allosteric response of tetracycline repressor. PNAS. 106: 22263-22268, 2009.
+ Publications citing the use of TetR (cat# P-1002):
1. Niederholtmeyer H et al. Real-Time mRNA Measurement during an in Vitro Transcription and Translation Reaction Using Binary Probes. ACS Synth. Biol., Publication Date (Web): December 19, 2012 (DOI: 10.1021/sb300104f)
2. Huang HH et al. Analysis of the leakage of gene repression by an artificial TetR-regulated promoter in cyanobacteria. BMC Res Notes. 2015 Sep 19;8:459. doi: 10.1186/s13104-015-1425-0.
* Please inquire for quotations for larger amounts
** Datasheet is available upon request.
