Recombinant Proteins
P-1004 Recombinant BlaR-carboxy-terminal domain from B. licheniformis (Rec. BlaR-CTD (Bl))The recombinant BlaR-carboxy-terminal domain from B. licheniformis (Rec. BlaR-CTD (Bl)) is a soluble form derived from B. licheniformis (a.a. 346-601 of GenBank accession no: P12287) with a N-terminal 6-his tag. It is expressed in bacteria and purified using Ni-affinity chromatography. It has a m.w. of approximately 30 kDa. Its purity is 98-99% based on SDS-PAGE analysis.
The Rec. BlaR-CTD (Bl) is functional in binding beta lactam including penicillins and cefalosporins as determined by ELISA, and lateral flow-based assay. It can be used as binding/capturing receptor in assays designed to quantitatively or semi-quantitatively measure beta lactam, such as competitive ELISA or immunochromatograph-based lateral flow.